Development of a yeast assay for rapid screening of inhibitors of human-derived Pneumocystis carinii dihydrofolate reductase.
نویسندگان
چکیده
Human-derived Pneumocystis carinii dihydrofolate reductase (DHFR) was expressed in a Saccharomyces cerevisiae strain whose growth depends on complementation by this enzyme. We utilized a quantitative assay to measure the sensitivity of this yeast strain to DHFR inhibitors. This assay should be useful for identifying new inhibitors of human-derived P. carinii DHFR.
منابع مشابه
Expression and characterization of recombinant human-derived Pneumocystis carinii dihydrofolate reductase.
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ورودعنوان ژورنال:
- Antimicrobial agents and chemotherapy
دوره 46 9 شماره
صفحات -
تاریخ انتشار 2002